Calcusyn v2
#CALCUSYN V2 SOFTWARE#
Here we use isobologram and combination-index data generated by CalcuSyn software analyses (Biosoft v2.1) to define drug interactivity in an objective, automated manner. The lack of reliable and standardised methodology to enable the in vitro definition of synergistic potential for antimalarials is a major drawback.
Identification of a synergistic partner drug would present an opportunity for dose-reduction, thus increasing the therapeutic window. Despite its 1000-fold increase in in vitro antimalarial potency (ED 50 47 nM) compared with its anti-amoebic potency (ED 50 26–32 uM), practical use of the compound has been limited by dose-dependent toxicity (emesis and cardiotoxicity). To fast-track antimalarial drug discovery, we have previously employed drug-repositioning to identify the anti-amoebic drug, emetine dihydrochloride hydrate, as a potential candidate for repositioned use against malaria. Unfortunately, antimalarial combination therapy is limited by the depleting repertoire of effective drugs with distinct target pathways. Combination therapies have a range of advantages, including synergism, toxicity reduction, and delaying the onset of resistance acquisition. Ileum- Simulation of experiments on the isolated Guinea-pig Ileum.The widespread introduction of artemisinin-based combination therapy has contributed to recent reductions in malaria mortality. QuantiScan- Densitometry using ordinary office scanners.ĬardioLab- Simulation of in vivo cardiovascular pharmacology experiments. UnGraph- Digitizer program which gives X,Y coordinates of lines or points on scannedĪssayZap- Universal assay calculator for RIA, ELISA, IRMA, colorimetric or any other typeĮnzFitter- Curve fitting and analysis of enzyme kinetics experiments. Stat-200- General statistics and graphics package. Already used in hundreds of academic labs and pharmaceutical companies, CalcuSyn is indispensable for the study of drug mixtures.įigSys- Publication quality graph plotting and data analysis. These give a thorough account of the theoretical basis of the analysis methods including the statistical treatment of results.
#CALCUSYN V2 PDF#
pdf with the software and there is a detailed Help file. There are flexible arrangements for printing of results and graphs and their export to spreadsheets, wordprocessors, graphics packages etc. All data and results are easily accessible by mouse-click on the contents tree.ĬalcuSyn Version 2.0 has Undo and Redo tools. The plots drawn by CalcuSyn include dose-effect, median-effect, isobolograms and CI-effect. Estimates of accuracy of EDx and CI can be calculated with Monte Carlo simulations or by a highly accurate algebraic estimation algorithm.
#CALCUSYN V2 PLUS#
CalcuSyn automatically graphs the data and produces reports giving summary statistics on all drugs plus detailed analysis of drug interactions including the Combination Index and EDx (for any value of x). When the grid is used, data can be processed through wizards which make the software easily accessible to new users.ĭata can be processed both for individual drugs and for constant-ratio or non-constant-ratio combinations of drugs. Data can be entered via the keyboard or file import either into the grid or directly into the analysis engine. 4, 450-454).ĬalcuSyn can easily be integrated with other software.
It performs multiple drug dose-effect calculations using the Median Effect methods described by T-C Chou and P. Mixed drug treatment is becoming common in the treatment of cancer, AIDS etc, and CalcuSyn is the most widely used software for establishing efficacy in this field.